When one is detecting nucleic acids by hybridization, there are numerous situations in which it is not desirable to isolate probe-target complexes from the excess of probes that are used. Sanjay Tyagi ...
Molecular beacons, single-stranded oligonucleotides that have a fluorescent "reporter" group at one end and a fluorescence "quencher" at the other, do not suffer from these shortcomings. 1 When free ...
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